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KMID : 0380619890210010164
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Korean Journal of Food Science and Technology
1989 Volume.21 No. 1 p.164 ~ p.172
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Formation of galactooligosaccharides by β - galactosidase from Streptococcus thermophilus 510
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Abstract
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The formation of galactooligosaccharides by transgalactosidation reactions during hydrolysis of lactose by the β-galactosidase from Streptococcus thermophilus 510 was investigated. Three oligosaccharides were detected during hydrolysis. It was found that the optimum conditions for the production of oligosaccharides was 40 lactose treated with β-galactosidase(50 ONPG units/ml) at 37℃ for 4 hours. The oligosaccharides formed accounted for 30% of the total sugars when the lactose had been 94% hydrolysed. 69% of the oligosaccharides were identified as 6-o--D -galactopyranosyl-D-glucose(allolactose) and 23% as 6-o-β-D-galactopyranosyl-D-galactose(isogalactobiose). The separation of galactooligosaccharides by the use of Bio-Gel P-2 gel permeation chromatography was also studied.
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